Human growth hormone as a reporter gene in regulation studies employing transient gene expression.
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چکیده
منابع مشابه
RT-PCR MEDIATED CLONING OF HUMAN GROWTH HORMONE GENE AND I TS EXPRESSION IN E. coli
Human growth hormone (hGH) genomic sequence containing 5 exons and 4 introns was cloned in pcDNA-3 and the constructed plasmid was subsequently used for transfection ofNlli-3T3 cell line using lipofection technique. Expression of hGH in stably transfected cells was assayed using ELISA. Total RNA was extracted from transfected cells and hGH cDNA was amplified by RT-PCR using specific primers...
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ژورنال
عنوان ژورنال: Molecular and Cellular Biology
سال: 1986
ISSN: 0270-7306,1098-5549
DOI: 10.1128/mcb.6.9.3173